Your Puppy’s Microbiome

The Universe inside your Puppy Here at My Pet Nutritionist we always focus on microbrial health, so we delve into it’s importance for your puppy to hopefully set them up for life. Microbes have been around for billions of years, humans – less than a million and we all know there is much disagreement over the domestication of our faithful furry friends. Microbes can multiply in minutes, survive and thrive in every habitat on earth, and technically, they’ve killed more people than all wars combined. But, without them, we actually couldn’t survive. Microbes are like a bad version of Ed Sheeran, you need me, I don’t need you. The body is in fact like a mini ecosystem. It has many different microbial communities throughout the body. They live inside; in the lungs, nose, urinary tract, and digestive tract, but they also live on; they are all over the skin! Because you sadly have jobs that need to get done today, we’re just going to do a whistle stop tour of the three main microbial communities and how we can support them in the puppy. First up, the skin microbiota. Not surprisingly, the skin microbiota plays a role in skin conditions like atopic dermatitis and even some skin cancers. Check out our blog on atopic dermatitis in pets here. In the dog, there are different communities found in different areas of the skin; there are also clear differences in diversity between healthy and allergic dogs. The skin provides one of the first lines of defence in the immune system, but in two ways. Not only does it have its physical structure to keep things in, and things out, but the community of microbes on the skin also protect against potentially harmful pathogens. The good guys can engulf the bad guys before they gain entry into the body, but they can also compete for nutrients and resources, to prevent the bad guys from thriving. In utero, foetal skin is thought to be sterile. But colonisation of microbes occurs during and immediately after birth. As the newborn puppy moves through the birth canal, he is exposed to a variety of bacteria from Mum. Once puppy is born and grooming begins, Mum passes even more over to her offspring. Mum health is therefore imperative – not only for the diversity she possesses but also her emotional health. Stressed Mum’s are less likely to engage in grooming behaviour, so if she isn’t grooming her offspring, she’s not passing her microbes to them. The environment greatly influences the microbiome of the skin. There are noted variations in skin microbial communities between those living rurally and those in urban areas. There is also an increase in chemical use associated with urban living, which also influences the composition of the microbiome. Diversity is significantly reduced with the use of detergents and antibacterial cleaning products. Potentially pathogenic taxa are also increased as there are fewer good guys to keep the bad guys in check. This is why skin issues like acne or dermatitis are deemed western diseases; they simply just aren’t found on the skin of indigenous tribes or on that of individuals from non-industrialised societies. What is also interesting is that there is also a clear distinction between male and female microbiome, therefore suggesting hormonal influences. This poses food for thought in the neutered pet and how their microbiome is subsequently affected. Unnecessary use of antibiotics also affects the skin microbiome, along with excessive use of grooming products and of course nutrition. The Oral Microbiota There is a collection of microbes found in the mouth; these are the ones that result in bad breath or dental issues. Again, they pose a first line of defence against ingested potentially harmful pathogens. But they also play a role in metabolising certain nutrients. It is clear that the oral microbiota found in dogs differs significantly from humans – some researchers have even gone as far as saying that a human bite would be more dangerous than a dog bite in terms of wound infection potential. Findings here The oral microbiota is gaining more attention and for good reason – there are associations between oral microbiota composition and weight gain, much like we have with the gut microbiota. So, caring for the mouth cavity is just as important as caring for the gut, and colonisation, like in the skin occurs at and shortly after birth – and certainly within the teething period! Data is relatively new, but in human realms, to support oral health, the guidelines regularly include avoiding ultra-processed foods along with high-sugar foods (think high-fructose corn syrup found in many dog treats and processed foods). Guidelines also promote dental hygiene –for our puppies and dogs, raw, meaty bones are a great opportunity to support dental health. Remember to choose appropriately sized bones for puppies – soft bones like chicken necks or wings. They must always be raw – cooked bones pose a splinter risk! The Gut Microbiota/Microbiome When we reference the microbiome, we are considering the microbial community found in the whole of the digestive tract. Generally, the further down we go, the more bugs we find. SIBO or small intestinal bacterial overgrow this when there are too many bugs in the small intestine. We want the majority of them in the large intestine. Each puppy and dog have a unique microbiome – just as we do. It’s like a fingerprint. How cool is that? But it just goes to show that if there is dysbiosis (imbalance of good to bad guys) – there is no one silver bullet. For our puppy, we are in a great position to support optimal gut health from the beginning (not withstanding gene interactions). When we talk about the microbiota or microbiome, we are not just talking about bacteria, but fungi and viruses which live inside the gut too. This is totally normal – the good guys with the right tools can keep the bad guys in check. This community can metabolise nutrients (ruminant
Puppy Nutrition 101

Puppy Nutrition 101 The dog is unique amongst other mammals in that it has the widest range of normal adult body weight within one species. Many breeds will reach 50% of their adult size within 5-6 months. Some breeds will be fully grown by 8-12 months, some 12-18 months, and others 18-24 months. With this incredible range, it’s no surprise that puppyhood is in fact a very sensitive period, and one that we need to get right. Here at My Pet Nutritionist, we are incredibly lucky that we support many owners in choosing the right nutritional plan for their growing puppy. But we thought we’d compile a brief guide to some of the most important things to consider for the growing puppy. Growth and Development After nursing, post-weaning growth is the most nutritionally demanding period in a dog’s life. For large and giant breeds, the length and speed of their growth poses an even higher nutritional demand. By maturity, most dogs will have increased their birth weight by 40-50 times. How amazing is this? But it also poses something to be very conscious of. Physical Growth How are bones formed? Also known as ossification, bone formation is a process where new bone is produced. Look at any large breed and it is clear to see how much new bone needs to be produced from birth to full skeletal maturity. Bone starts as a cartilage model which gets slowly replaced. Osteoblasts are the cells that form new bone. They secrete osteoids. Osteoid tissue is simply unmineralised bone tissue. Soon after the osteoid is laid down,inorganic salts are deposited which forms the hardened material that we know as bone. These inorganic salts include calcium and phosphorus. Bone formation proceeds outwards from ossification centres; short bones tend to have one in the middle whereas long bones typically have three, one at each end and one in the middle. Ossification, or bone formation continues until there is a thin strip of cartilage left at each end of the bone. This is known as the epiphyseal plate, or growth plate in the dog world. When the bone reaches full adult maturity, the cartilage is replaced with bone and“sealed” for want of a better word. It is therefore no surprise that certain minerals are necessary for physical bone growth, along with some vitamins. Calcium is the most abundant mineral in the body; the majority of which is contained in the skeleton. Phosphorus is the next most abundant; also found in bone. Although they have separate functions within the body, being so closely interrelated, they are usually considered together. Calcium is involved in constriction and dilation of blood vessels, nerve impulse transmission, muscle contractions(including the heart), secretion of hormones and blood coagulation. It also plays a role in fluid balance in cells (contributing to homeostasis). A diet grossly inadequate in calcium, results in hypocalcemia. The body will remove calcium from bones to ensure normal cell function in the rest of the body. This leads to weakened bones. Hypercalcemia is a little more uncommon, but it is most often linked with overactive parathyroid gland function. Phosphorus is essential in cell growth and differentiation, energy use and transfer, fatty acid transport and amino acid and protein formation. In general, phosphorus is better absorbed from meat products than plant products. Phosphorus found in meat is mainly in its organic form; in plants it is in the form of phytic acid. Phytic acid impairs the absorption of iron,zinc and calcium and has been linked to mineral deficiencies. It is often referred to as an anti-nutrient. Deficiencies of calcium and phosphorus are unusual, but imbalance is noted. In unbalanced diets, calcium deficiency can develop alongside high levels of phosphorus. Low calcium levels stimulate the release of parathyroid hormone, which increases the production of the active form of Vitamin D. This results in an increased bone reabsorption to restore calcium levels. This eventually leads to bone demineralisation and a loss of bone mass. In dogs, this is more commonly noticed in the mandibles (jaw bones), leading to tooth loss and periodontal disease. Attention is most often paid to the calcium:phosphorus ratio. The note to make is that organic sources of both minerals are slowly and less efficiently absorbed; so, in fresh fed dogs this is less of a concern in terms of excess. Phosphorus found in meat products however is more efficiently absorbed than that found in plants. Findings here Other nutrients relevant to skeletal development include vitamin D, vitamin A, copper, zinc and manganese. Deficiency or excess in these have regularly been linked to abnormal orthopaedic development. But, as we know, it is not just the bones in puppies that grow – it is in fact every part of their body! What do puppies need to grow? Protein Proteins are large, complex molecules composed of hundreds to thousands of amino acids. They are literally the building blocks of the body. Protein is required in the diet to provide a source of amino acids to build, repair and replace body proteins. Proteins in the body have numerous functions. Major structural components of hair, skin, nails, tendons, ligaments and cartilage. Hormones are composed of protein molecules – these include insulin and glucagon which are key to maintaining blood sugar levels. Proteins are found in the blood– for example haemoglobin carries oxygen between the lungs and cells,lipoproteins carry fats throughout the body and transferrin carries iron through the blood. Proteins are also found in the immune system in the form of immunoglobulins to make the antibodies that provide resistance to disease. All proteins are in a constant state of renewal and degradation and during growth (or reproduction) additional protein is needed for the creation of new tissue. High rates of protein synthesis occur in: The production of red and white blood cells Epithelial cells of the skin and those lining the GI tract and pancreas Digestion of Protein Dietary amino acids are absorbed in the gastrointestinal tract, following disruption of their structure. They are then transported